Parameters for F:\Projects\MedChem\KellyLee\Sally\18Feb2020_NA.PRO\ACQUDB\mobileHDX_15min_DRE.EXP Created by 4.2 SCN1003 Lock Spray Configuration: Reference Scan Frequency(sec) 60.000 Reference Cone Voltage(V) 40.000 Reference Trap Collision Energy 21.000 Reference DRE Setting 99.900 Temperature Correction: Temperature Correction Disabled Instrument Configuration: Lteff 1800.0 Veff 7199.20 Resolution 20000 Min Points in Peak 2 Acquisition Device WatersADC Acquisition Algorithm ADC Mode ADC Trigger Threshold (V) 1.00 ADC Input Offset (V) -1.50 Average Single Ion Intensity 30 ADC Amplitude Threshold 3 ADC Centroid Threshold -1 ADC Ion Area Threshold 3 ADC Ion Area Offset 10 ADC Pushes Per IMS Increment 1 EDC Delay Coefficient 1.3500 EDC Delay Offset 0.4000 Experimental Instrument Parameters Instrument Parameter Filename F:\Projects\MedChem\KellyLee\Sally\18Feb2020_NA.PRO\ACQUDB\HDX_70_130_Accurate_HDX_2018_3.IPR (MODIFIED) Polarity ES+ Capillary (kV) 2.5000 Source Temperature (°C) 70 Sampling Cone 40.0000 Source Offset 50.0000 Source Gas Flow (mL/min) 0.00 Desolvation Temperature (°C) 130 Cone Gas Flow (L/Hr) 80.0 Desolvation Gas Flow (L/Hr) 600.0 Nebuliser Gas Flow (Bar) 6.5 LM Resolution 4.0 HM Resolution 15.0 Aperture 1 0.0 Pre-filter 2.0 Ion Energy 1.0 Manual Trap Collision Energy FALSE Trap Collision Energy 4.0 Manual Transfer Collision Energy FALSE Transfer Collision Energy 2.0 Manual Gas Control FALSE Trap Gas Flow (mL/min) 2.00 HeliumCellGasFlow 180.00 IMS Gas Flow (mL/min) 90.00 Detector 2400 DetectorCache 2150 Sample Infusion Flow Rate (µL/min) 20 Sample Flow State LC Sample Fill Volume (µL) 250 Sample Reservoir Wash LockSpray Infusion Flow Rate (µL/min) 20 LockSpray Flow State Infusion LockSpray Reservoir B LockSpray Capillary (kV) 2.0 Use Manual LockSpray Collision Energy FALSE Collision Energy 4.0 Acceleration1 67.0 Acceleration2 200.0 Aperture2 70.0 Transport1 70.0 Transport2 70.0 Steering 1.02 Tube Lens 74 Pusher 1889.0 Pusher Offset -0.38 Puller 1361.0 Pusher Cycle Time (µs) Automatic Pusher Width (µs) Automatic Collector 60 Collector Pulse 10.0 Stopper 10 Stopper Pulse 20.0 Entrance 62 Static Offset 180 Puller Offset -0.00 Reflectron Grid (kV) 1.478 Flight Tube (kV) 10.00 Reflectron (kV) 3.780 Use Manual Trap DC TRUE Trap DC Entrance 3.0 Trap DC Bias 45.0 Trap DC 0.0 Trap DC Exit 3.0 Use Manual IMS DC TRUE IMS DC Entrance 20.0 Helium Cell DC 50.0 Helium Exit -20.0 IMSBias 3.0 IMS DC Exit 0.0 USe Manual Transfer DC TRUE Transfer DC Entrance 4.0 Transfer DC Exit 15.0 Trap Manual Control OFF Trap Wave Velocity (m/s) 311 Trap Wave Height (V) 4.0 IMS Manual Control ON IMS Wave Velocity (m/s) 650 IMS Wave Height (V) 35.0 Transfer Manual Control ON Transfer Wave Velocity (m/s) 380 Transfer Wave Height (V) 4.0 Step Wave 1 In Manual Control ON Enable Reverse Operation OFF Step Wave 1 In Velocity (m/s) 300.0 Step Wave 1 In Height 10.0 Step Wave 1 Out Manual Control ON Step Wave 1 Out Velocity (m/s) 300.0 Step Wave 1 Out Height 0.0 Step Wave 2 Manual Control ON Step Wave 2 Velocity (m/s) 300.0 Step Wave 2 Height 0.0 Use Manual Step Wave DC ON Step Wave TransferOffset 10.0 Step Wave DiffAperture1 0.0 Step Wave DiffAperture2 0.0 Use Automatic RF Settings FALSE StepWave1RFOffset 100.0 StepWave2RFOffset 100.0 Target Enhancement Enabled FALSE Target Enhancement Mode EDC Target Enhancement Mass 556.0 Target Enhancement Trap Height (V) 12.0 Target Enhancement Extract Height (V) 8.0 Mobility Trapping Manual Release Enabled FALSE Mobility Trapping Release Time (µs) 500 Mobility Trap Height (V) 15.0 Mobility Extract Height (V) 0.0 Trag Gate LUT table enabled FALSE TriWave Trap Gate LookUp Table Using Drift Time Trimming TRUE Drift Time Bins 0 Using Mobility Delay after Trap Release TRUE IMS Wave Delay (µs) 1000 Variable Wave Height Enabled FALSE Wave Height Ramp Type Linear Wave Height Start (V) 10.0 Wave Height End (V) 40.0 Wave Height Using Full IMS TRUE Wave Height Ramp (%) 100.0 Wave Height Look Up Table Variable Wave Velocity Enabled TRUE Wave Velocity Ramp Type Linear Wave Velocity Start (m/s) 600.0 Wave Velocity End (m/s) 1000.0 Wave Velocity Using Full IMS TRUE Wave Velocity Ramp (%) 100.0 Wave Velocity Look Up Table Backing 3.31e0 Source 9.00e-3 Sample Plate 1.00e-6 Trap 2.71e-2 Helium Cell 4.59e0 IMS 3.00e0 Transfer 2.81e-2 TOF 7.41e-7 IMSRFOffset 300 IMSMobilityRFOffset 250 TrapRFOffset 300 Use Automatic RF Settings FALSE AutoStepWave1RFOffset 150 AutoStepWave2RFOffset 150 TransferRFOffset 350 MS Profile Type Auto P MSProfileMass1 100 MSProfileDwellTime1 20 MSProfileRampTime1 20 MSProfileMass2 300 MSProfileDwellTime2 20 MSProfileRampTime2 40 MSProfileMass3 500 PusherInterval 69.000000 PusherOffset 0.250000 LockMassValidSigma 5 Acquisition mass range Start mass 150.000 End mass 2000.000 Calibration mass range Start mass 91.062 End mass 1925.941 Experiment Reference Compound Name: Leucine Enkephalin Function Parameters - Function 1 - MOBILITY MS FUNCTION Scan Time (sec) 2.000 Interscan Time (sec) 0.015 Start Mass 150.0 End Mass 2000.0 Start Time (mins) 3.44 End Time (mins) 19.00 Data Format Continuum Analyser Resolution Mode ADC Sample Frequency (GHz) 3.0 ADC Pusher Frequency (µs) 69.0 ADC Pusher Width (µs) 1.75 Use Tune Page Cone Voltage YES Using Auto Trap Collision Energy (eV) 4.000000 Using Auto Transfer Collision Energy (eV) 2.000000 Sensitivity Normal Dynamic Range Normal Save Collapsed Retention Time Data No Use Rule File Filtering No FragmentationMode CID Calibration Dynamic 2 Function Parameters - Function 2 - REFERENCE Scan Time (sec) 1.000 Interscan Time (sec) 0.100 Start Mass 150.0 End Mass 2000.0 Start Time (mins) 3.44 End Time (mins) 19.00 Data Format Continuum Analyser Resolution Mode ADC Sample Frequency (GHz) 3.0 ADC Pusher Frequency (µs) 69.0 ADC Pusher Width (µs) 1.75 Use Tune Page Cone Voltage YES Using Auto Trap Collision Energy (eV) 4.000000 Using Auto Transfer Collision Energy (eV) 2.000000 Sensitivity Normal Dynamic Range Normal Save Collapsed Retention Time Data No Use Rule File Filtering No FragmentationMode CID [LOCK SPRAY] LockSprayType: Do not apply correction LockSpray Reference Compound Name: Leucine Enkephalin Scan Time (sec) 1.00 Interval 60 EDC Mass 0.0000 Mass Window +/- 0.4 Scans to Average 1.0 Reference Cone Voltage 40.0 Trap Collision Energy (eV) 21.0 LocksprayDRESetting 99.9000 [METHOD EVENTS:] Timed Events Enabled: YES API Probe Delay Temp: 20.0 Num of Timed Events: 5.0 Event Time Name Action System 1 -60.00 Flow State LC Sample 2 -60.00 Reservoir B LockSpray 3 -60.00 Refill Auto-Refill LockSpray 4 -60.00 Infusion Start LockSpray 5 60000.00 Infusion Stop LockSpray Calibration Dynamic 2