Parameters for E:\projects AG Kammerer\Simon\Simon.PRO\ACQUDB\20180808_NuclIMS.EXP Created by 4.1 SCN916 Lock Spray Configuration: Reference Scan Frequency(sec) 30.000 Reference Cone Voltage(V) 40.000 Reference Trap Collision Energy 4.000 Reference DRE Setting 99.900 Temperature Correction: Temperature Correction Disabled Instrument Configuration: Lteff 1800.0 Veff 7195.30 Resolution 20000 Min Points in Peak 2 Acquisition Device WatersADC Acquisition Algorithm ADC Mode ADC Trigger Threshold (V) 0.90 ADC Input Offset (V) -1.59 Average Single Ion Intensity 33 ADC Amplitude Threshold 3 ADC Centroid Threshold -1 ADC Ion Area Threshold 4 ADC Ion Area Offset 10 ADC Pushes Per IMS Increment 1 EDC Delay Coefficient 1.3500 EDC Delay Offset 0.4000 Experimental Instrument Parameters Instrument Parameter Filename E:\projects AG Kammerer\Simon\Simon.PRO\ACQUDB\Nucleoside_IMS_opti.IPR (MODIFIED) Polarity ES+ Capillary (kV) 2.5000 Source Temperature (°C) 110 Sampling Cone 20.0000 Source Offset 20.0000 Source Gas Flow (mL/min) 0.00 Desolvation Temperature (°C) 600 Cone Gas Flow (L/Hr) 40.0 Desolvation Gas Flow (L/Hr) 1200.0 Nebuliser Gas Flow (Bar) 6.0 LM Resolution 10.0 HM Resolution 15.0 Aperture 1 0.0 Pre-filter 2.0 Ion Energy 1.0 Manual Trap Collision Energy TRUE Trap Collision Energy 4.0 Manual Transfer Collision Energy TRUE Transfer Collision Energy 2.0 Manual Gas Control TRUE Trap Gas Flow (mL/min) 0.40 HeliumCellGasFlow 180.00 IMS Gas Flow (mL/min) 70.00 Detector 2925 DetectorCache 0 Sample Infusion Flow Rate (µL/min) 5 Sample Flow State LC Sample Fill Volume (µL) 250 Sample Reservoir A LockSpray Infusion Flow Rate (µL/min) 5 LockSpray Flow State Infusion LockSpray Reservoir B LockSpray Capillary (kV) 1.5 Use Manual LockSpray Collision Energy FALSE Collision Energy 4.0 Acceleration1 70.0 Acceleration2 200.0 Aperture2 70.0 Transport1 70.0 Transport2 70.0 Steering 0.00 Tube Lens 70 Pusher 1900.0 Pusher Offset -0.40 Puller 1370.0 Pusher Cycle Time (µs) Automatic Pusher Width (µs) Automatic Collector 50 Collector Pulse 10.0 Stopper 10 Stopper Pulse 20.0 Entrance 62 Static Offset 180 Puller Offset 0.00 Reflectron Grid (kV) 1.470 Flight Tube (kV) 10.00 Reflectron (kV) 3.780 Use Manual Trap DC TRUE Trap DC Entrance 3.0 Trap DC Bias 45.0 Trap DC 0.0 Trap DC Exit 3.0 Use Manual IMS DC TRUE IMS DC Entrance 35.0 Helium Cell DC 35.0 Helium Exit -5.0 IMSBias 3.0 IMS DC Exit 0.0 USe Manual Transfer DC TRUE Transfer DC Entrance 4.0 Transfer DC Exit 15.0 Trap Manual Control OFF Trap Wave Velocity (m/s) 300 Trap Wave Height (V) 6.0 IMS Manual Control ON IMS Wave Velocity (m/s) 650 IMS Wave Height (V) 30.0 Transfer Manual Control ON Transfer Wave Velocity (m/s) 315 Transfer Wave Height (V) 4.0 Step Wave 1 In Manual Control OFF Enable Reverse Operation OFF Step Wave 1 In Velocity (m/s) 300.0 Step Wave 1 In Height 10.0 Step Wave 1 Out Manual Control OFF Step Wave 1 Out Velocity (m/s) 300.0 Step Wave 1 Out Height 0.0 Step Wave 2 Manual Control OFF Step Wave 2 Velocity (m/s) 300.0 Step Wave 2 Height 0.0 Use Manual Step Wave DC ON Step Wave TransferOffset 10.0 Step Wave DiffAperture1 3.0 Step Wave DiffAperture2 0.0 Use Automatic RF Settings FALSE StepWave1RFOffset 80.0 StepWave2RFOffset 350.0 Target Enhancement Enabled FALSE Target Enhancement Mode EDC Target Enhancement Mass 556.0 Target Enhancement Trap Height (V) 12.0 Target Enhancement Extract Height (V) 8.0 Mobility Trapping Manual Release Enabled FALSE Mobility Trapping Release Time (µs) 500 Mobility Trap Height (V) 20.0 Mobility Extract Height (V) 0.0 Trag Gate LUT table enabled FALSE TriWave Trap Gate LookUp Table Using Drift Time Trimming FALSE Drift Time Bins 0 Using Mobility Delay after Trap Release FALSE IMS Wave Delay (µs) 1000 Variable Wave Height Enabled FALSE Wave Height Ramp Type Linear Wave Height Start (V) 17.0 Wave Height End (V) 35.0 Wave Height Using Full IMS TRUE Wave Height Ramp (%) 50.0 Wave Height Look Up Table Variable Wave Velocity Enabled FALSE Wave Velocity Ramp Type Linear Wave Velocity Start (m/s) 1000.0 Wave Velocity End (m/s) 400.0 Wave Velocity Using Full IMS TRUE Wave Velocity Ramp (%) 50.0 Wave Velocity Look Up Table Backing 3.01e0 Source 7.38e-3 Sample Plate 1.00e-6 Trap 1.58e-2 Helium Cell 3.07e0 IMS 2.65e0 Transfer 1.77e-2 TOF 4.14e-7 IMSRFOffset 300 IMSMobilityRFOffset 150 TrapRFOffset 300 Use Automatic RF Settings FALSE AutoStepWave1RFOffset 300 AutoStepWave2RFOffset 350 TransferRFOffset 350 MS Profile Type Auto P MSProfileMass1 100 MSProfileDwellTime1 20 MSProfileRampTime1 20 MSProfileMass2 300 MSProfileDwellTime2 20 MSProfileRampTime2 40 MSProfileMass3 500 PusherInterval 39.000000 PusherOffset 0.250000 LockMassValidSigma 5 Acquisition mass range Start mass 50.000 End mass 600.000 Calibration mass range Start mass 90.996 End mass 1178.607 Experiment Reference Compound Name: LeuEnk_SP_Res_pos Function Parameters - Function 1 - MOBILITY MS FUNCTION Scan Time (sec) 0.500 Interscan Time (sec) 0.014 Start Mass 50.0 End Mass 600.0 Start Time (mins) 0.00 End Time (mins) 12.00 Data Format Continuum Analyser Resolution Mode ADC Sample Frequency (GHz) 3.0 ADC Pusher Frequency (µs) 39.0 ADC Pusher Width (µs) 1.00 Use Tune Page Cone Voltage YES Using Auto Trap Collision Energy (eV) 4.000000 Using Auto Transfer Collision Energy (eV) 2.000000 Sensitivity Normal Dynamic Range Normal Save Collapsed Retention Time Data Yes Use Rule File Filtering No FragmentationMode CID Calibration Dynamic 2 Function Parameters - Function 2 - MOBILITY MS FUNCTION Scan Time (sec) 0.500 Interscan Time (sec) 0.014 Start Mass 50.0 End Mass 600.0 Start Time (mins) 0.00 End Time (mins) 12.00 Data Format Continuum Analyser Resolution Mode ADC Sample Frequency (GHz) 3.0 ADC Pusher Frequency (µs) 39.0 ADC Pusher Width (µs) 1.00 Use Tune Page Cone Voltage YES Using Auto Trap Collision Energy (eV) 4.000000 Use Auto Transfer Collision Energy NO Transfer Collision Energy Ramp Start (eV) 20.0 Transfer Collision Energy Ramp End (eV) 50.0 Sensitivity Normal Dynamic Range Normal Save Collapsed Retention Time Data Yes Use Rule File Filtering No FragmentationMode CID Calibration Dynamic 2 Function Parameters - Function 3 - REFERENCE Scan Time (sec) 0.000 Interscan Time (sec) 0.100 Start Mass 50.0 End Mass 600.0 Start Time (mins) 0.00 End Time (mins) 12.00 Data Format Continuum Analyser Resolution Mode ADC Sample Frequency (GHz) 3.0 ADC Pusher Frequency (µs) 39.0 ADC Pusher Width (µs) 1.00 Use Tune Page Cone Voltage YES Using Auto Trap Collision Energy (eV) 4.000000 Using Auto Transfer Collision Energy (eV) 2.000000 Sensitivity Normal Dynamic Range Normal Save Collapsed Retention Time Data Yes Use Rule File Filtering No FragmentationMode CID [LOCK SPRAY] LockSprayType: Do not apply correction LockSpray Reference Compound Name: LeuEnk_SP_Res_pos Scan Time (sec) 0.00 Interval 30 EDC Mass 0.0000 Mass Window +/- 0.5 Scans to Average 3.0 Reference Cone Voltage 40.0 Trap Collision Energy (eV) 4.0 LocksprayDRESetting 99.9000 [METHOD EVENTS:] Timed Events Enabled: YES API Probe Delay Temp: 20.0 Num of Timed Events: 4.0 Event Time Name Action System 1 -60.00 Flow State LC Sample 2 -60.00 Reservoir B LockSpray 3 -60.00 Refill Auto-Refill LockSpray 4 -60.00 Infusion Start LockSpray Calibration Dynamic 2